Mastering Nanomedicines Characterization: Techniques for Determining Particle Size Distribution Based on Dynamic Light Scattering

The determination of particle size distribution (PSD) of drug nanocarriers is crucial for their successful development and use. Dynamic light scattering (DLS) is a commonly used technique for measuring PSD, and it provides information on the hydrodynamic size of particles by analyzing the fluctuations in scattering intensity over time. This webinar will cover the principles of DLS, including cumulants and regularization analysis, as well as the limitations of the technique, such as low resolution and accuracy for very polydisperse samples.

The webinar will also discuss multi-angle DLS, which can provide better resolution by measuring in forward, side, and backscattering angles, and spatially-resolved DLS, which is based on low coherence interferometry and can measure high concentrations of nanoparticles in flow or in containers. The application of DLS in the analysis of polystyrene size standards, mixtures of size standards, and drug products such as liposomes will be presented, along with the use of asymmetrical flow field-flow fractionation (AF4) in conjunction with DLS to measure PSD.

​​​​​​​Attendees of this webinar will gain a thorough understanding of the principles and techniques based on dynamic light scattering for determining the PSD of drug nanocarriers. They will learn about the advantages and limitations of these techniques, and gain insight into how to apply them to different types of drug nanocarriers. The webinar will provide attendees with valuable information and tools to help them optimize the development and use of nanomedicines in a variety of applications.

Presenter

Maria Marioli, PhD

Senior Scientist – Group Lead
Nanomedicine Analytical Chemistry


Maria holds a PhD in Analytical Chemistry from the University of Amsterdam and she is specialized in the characterization of large molecules and nanoparticles for pharmaceutical applications.  After working for several years in large pharmaceutical companies, Maria joined Ardena and she responsible for the analytical method development and validation of nanomedicine projects.​​​​​​​